![]() ![]() ![]() Close the Threshold dialog window without clicking any of the buttons. How to select pixels by color in ImageJ I want to use ImageJ for counting my different tephra populations in a thin section sample, but I am not able to separate the lighter populations from. Adjust the sliders so that the dark, low-value pixels that represent water turn red, but those that represent land don't change. The Threshold dialog window allows you to highlight pixels in an image that have values within a range you define. This action converts each pixel's color information into a brightness measurement. Choose Image > Type > 8-bit to convert the image to grayscale.NOTE: the techniques described here do not work as well on the MODIS images that you used in the previous section, as there is not enough contrast in the MODIS images. As the ranges of pixel values that represent land and water are unique, you can manipulate ImageJ to highlight the pixels that represent water in each image, and measure the area it covers. If the color information for each pixel is converted to grayscale, the dark pixels representing water will have low values and the lighter pixels that represent land will have higher values. I want to go through each ROI, and set to zero any pixel which is above a certain intensity value (very bright pixels). Then segment cells using thresholding and morpholibJ, resulting in 1 ROI per cell. In these images, pixels that represent water are much darker than pixels that represent land. I am imaging nuclear pores in widefield with fluorescent dyes. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |